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Old 11-05-2013, 01:39 PM   #1
Location: Milwaukee

Join Date: Dec 2011
Posts: 72
Default Evaluating overdispersion in CummeRbund


I am analyzing RNA-Seq data in a TopHat/Cufflinks/CummeRbund pipeline. In the CummeRbund manual, it is recommended that researchers check for overdispersion for each sample as a quality control. How does one tell if a sample is overdispersed? Is there a statistical test that can quantify overdispersion?

I have attached some of my dispersion plots for gene expression. Is there any way to tell if they are overdispersed?
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File Type: pdf dispersionPlots.pdf (2.27 MB, 50 views)
gwilymh is offline   Reply With Quote

cuffdiff, cummerbund, cummerbund plots, tophat

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