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Old 09-20-2016, 12:17 PM   #1
starbug
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Location: london

Join Date: Nov 2013
Posts: 5
Default Quality per tile

Hi there,

I have three samples which were sequenced on a MiSeq. I have attached the R1 and R2 quality per tile for sample 3 and R2 for samples 1 and 2.

For the reverses I get a quality per tile fail for R2 with almost identical plots for all three samples.

After trimming (which normally works pretty well!!) things seem worse.

Is there something else I should be doing (e.g. re-trimming/removing affected reads)?

Any help/advice would be great!!! Thanks in advance!!!
Attached Images
File Type: png Quality per tile.png (87.8 KB, 19 views)
File Type: png Quality per tile - sample 2.png (87.8 KB, 7 views)
File Type: png Quality per tile - sample 3.png (87.5 KB, 4 views)
File Type: png Quality per tile - sample 3 - forward.png (82.4 KB, 7 views)
File Type: png sample 3 R2 post trimming.png (85.8 KB, 12 views)
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illimina miseq, miseq 250bp, quality per tile

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