FPKM/TPM to raw read counts???????

[jpg2006]

Hey guys,

I am new to this. My question is if there is any way(s) to convert TPM into raw read counts? I currently have FPKM, TPM,effective length and length of the genes for my data set. If I understand correctly I could be able to multiply the FPKM values by the length of the gene, but that would be an incredible amount of manual work due to how the data I have is set up. So besides this way is it possible to convert the data I have into raw read counts? I intend to do other normalizations with the raw read counts because I am aware that TPM and FPKM are already normalized outputs.

I apologize if this is unclear, though any advice would be great! If it helps I am currently using data from experiments I didn't conduct, so the FPKM, TPM, effective length of genes, and the length of the genes are all I have.

Thank you!

[SamCurt]

Please check the Bioconductor package tximport, which reverse-calculate read counts from FPKM/TPM, with two modes.