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Old 06-01-2011, 02:00 AM   #1
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Default PubMed: Rapid screening of complex DNA samples by single-molecule amplification and s

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Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

PLoS One. 2011;6(5):e19723

Authors: Huang J, Zheng Z, Andersson AF, Engstrand L, Ye W

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.

PMID: 21625543 [PubMed - in process]

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