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Hi everyone,
I wonder if some of you have used edgeRun for RNA-Seq data. I had to because the datasets have high biological variability (BCV ~ 0.4) as they come from genetically independent animals and the sample numbers are low (4 animals, two tissues each).
The manual recommends using at least 50000 iterations for the test. However, I'm not that good at maths to know what are the implications of higher or lower iterations in a test of this kind, to know whether should I do it on 50000 or higher number of iterations.
Anyone could help?
Thanks in advance =) always big help.
I wonder if some of you have used edgeRun for RNA-Seq data. I had to because the datasets have high biological variability (BCV ~ 0.4) as they come from genetically independent animals and the sample numbers are low (4 animals, two tissues each).
The manual recommends using at least 50000 iterations for the test. However, I'm not that good at maths to know what are the implications of higher or lower iterations in a test of this kind, to know whether should I do it on 50000 or higher number of iterations.
Anyone could help?
Thanks in advance =) always big help.