Hi there,
I got samples from the brain nucleus RNA and sequenced it using PacBio RS2. However, Mitochondria exist only in the cytoplasm,why my data mapped to chrM?(screenshot shown on below) Does my samples of nucleus RNA contaminate cytoplasmic RNA?
Meanwhile, i want to know weather exist the difference between cytoplasmic RNA and nucleus RNA for the same mRNA?
I got samples from the brain nucleus RNA and sequenced it using PacBio RS2. However, Mitochondria exist only in the cytoplasm,why my data mapped to chrM?(screenshot shown on below) Does my samples of nucleus RNA contaminate cytoplasmic RNA?
Meanwhile, i want to know weather exist the difference between cytoplasmic RNA and nucleus RNA for the same mRNA?