Would like to understand something when I use Covaris for shearing.
I have DNA that has been diluted to 5ug in 120ul for shearing or 41ng/ul. I shear and measure concentration by Picogreen. But it is about 30ng/ul after shearing. Why does the concentration drop? It is only sheared DNA, not cleaned. Is it due to the DNA being less compact after shearing? Does it have anything to do that DNA is fragmented now and this causes the concentration to drop? I have tried other DNAs and their concentrations drop just like this.
I have DNA that has been diluted to 5ug in 120ul for shearing or 41ng/ul. I shear and measure concentration by Picogreen. But it is about 30ng/ul after shearing. Why does the concentration drop? It is only sheared DNA, not cleaned. Is it due to the DNA being less compact after shearing? Does it have anything to do that DNA is fragmented now and this causes the concentration to drop? I have tried other DNAs and their concentrations drop just like this.
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