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  • Reagent v3 Miseq stability

    Hello everyone!

    I left reagent V3 in water bath at room temperature for roughly 3 hours. I got a low Q30 score and the cluster density was 1050 K/mm2.

    Do you think that the thawing process could affect?

    Thanks for your answers!

  • #2
    I kept the reagent cartridge in water bath for almost 2 hr and it was OK.
    The cluster density 1050 K/mm2 is in normal range.
    I think the quality of your sample might not good that's why you got low Q30 score. So, I don't think 3 hrs thawing might affect your seq.

    Can you share your bioanalyzer result?

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