Hello,
I have RNA concentration around 15-18 ng/ul (260/280 is 1.5-1.8 but 260/230 is very low ~0.5) on which I need to do polyA library preparation and sequencing. The company said you need to purify the samples. If I purify the samples, I will lose more RNA. Is it risky to go ahead with library preparation without purifying the samples?
Thanks
I have RNA concentration around 15-18 ng/ul (260/280 is 1.5-1.8 but 260/230 is very low ~0.5) on which I need to do polyA library preparation and sequencing. The company said you need to purify the samples. If I purify the samples, I will lose more RNA. Is it risky to go ahead with library preparation without purifying the samples?
Thanks
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