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  • What volume of adapters do you get in the nextera XT kits?

    Hello,

    At our lab we have a custom protocol for adding indices to our amplicons, using only 5uL of each index when using the "Nextera XT Index Kit (24 indexes, 96 samples)".

    I want to update this to use the "IDT® for Illumina® Nextera™ DNA UD Indexes Set A (96 Indexes, 96 Samples)" kit, but I can't find out how much (approximately) you get in each kit for each pair of indices.

    Has anyone bought this kit?

  • #2
    10 ul with some overage in each well which contains pool of two primers.

    Comment


    • #3
      Thank you, most appreciated.

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