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There is definitely a variability between the SV and LV kits. With the new LV oil, I see a distinct clear layer after the emulsion PCR, but with SV, there is not a clear layer in all of them. Not sure if the cpb are transferable from SV to LV. Only thing I suggest is to aim low when it comes to cpb and CAREFULLY detect the broken emulsions (I explained how I do this above) and make sure to take them out before breaking.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
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