The Ribo-Zero™ Gold Kit (Human/Mouse/Rat) is an enhanced version of Epicentre's popular Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat). The Ribo-Zero Gold Kit removes both cytoplasmic (nuclear-encoded) rRNA and mitochondrial rRNA from 1 to 5 µg of human, mouse, or rat total RNA preparations for RNA-Seq and other applications. For more information, please see the product page.
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The Ribo-Zero method will allow for purification of microRNA, as long you use a suitable clean-up method at the end. The ScriptSeq kit is not designed for microRNA library prep; instead, we recommend using our ScriptMiner small RNA-seq kit.
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yes, I realize that you have specific preps for microRNA like Illumina has, but this would call for two separate preparations for the same samples(s). I just wanted to know that even though the ScriptSeq is not designed for microRNA, whether the microRNA could be included, either by the standard workflow or by some modification. It seems the small/micro RNA would presumably be lost in the ScriptSeq workflow during purification steps, but if there is an alternative purification method perhaps it could be included?
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The yields are similar for the standard Ribo-Zero and the Ribo-Zero Gold kits, i.e., ranging from 3% to 10% of input amount, depending on the type of total RNA sample.
The mapping was done using Genomatix RegionMiner. The default parameters are as follows:
In case the region classification has been checked, a table with the number of regions contained in genomic elements is given. Each input region is classified either as
exonic, complete or
exonic, partial (i.e. overlapping with an exon) or
intronic, complete or
intergenic region
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