Hi everybody,
I am looking at my Fastq QC stats with much surprise: 25% of my reads contain Trueseq adapter sequences. That seems like a lot. I am completely new to NGS data analysis, so I have no point of comparison... is this very unusual? What type of analysis should I do to find out more about the roots of this problem?
This is a 50bp PE run on a HiSeq 2000, target enrichment using Haloplex.
Any comments would be appreciated.
Thanks!
I am looking at my Fastq QC stats with much surprise: 25% of my reads contain Trueseq adapter sequences. That seems like a lot. I am completely new to NGS data analysis, so I have no point of comparison... is this very unusual? What type of analysis should I do to find out more about the roots of this problem?
This is a 50bp PE run on a HiSeq 2000, target enrichment using Haloplex.
Any comments would be appreciated.
Thanks!
Comment