Hi all,
I am preparing gDNA for Illumina sequening (short reads and paired-end). I will be quantifying the sample using qPCR. I am wondering why RNase treatment is recommended in some sample protocols and if I really need to do it. Could it potentially interfere with quantification by the genomics facility where I am sequencing? I would prefer not to RNase treat as I have limited DNA and I don't want to mess with it too much. DNA was extracted using a Qiagen kit and looks pretty pure. Could small amounts of RNA disrupt the library preparation process or the quantification process when I send the samples in for library prep?
Any help would be appreciated!
I am preparing gDNA for Illumina sequening (short reads and paired-end). I will be quantifying the sample using qPCR. I am wondering why RNase treatment is recommended in some sample protocols and if I really need to do it. Could it potentially interfere with quantification by the genomics facility where I am sequencing? I would prefer not to RNase treat as I have limited DNA and I don't want to mess with it too much. DNA was extracted using a Qiagen kit and looks pretty pure. Could small amounts of RNA disrupt the library preparation process or the quantification process when I send the samples in for library prep?
Any help would be appreciated!
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