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  • Time series issue,no time 0!

    Hi everybody
    I have encountered a problem during analysis of time series data.
    I particular, in my data, I have 2 condition(in replicates) and 4 time.
    drug 1h Placebo 1h
    drug 1h Placebo 1h
    drug 2h Placebo 2h
    drug 2h Placebo 2h
    drug 3h Placebo 3h
    drug 3h Placebo 3h
    drug 4h Placebo 4h
    drug 4h Placebo 4h
    As you can see I have no time 0 in my analysis and I was wondering if I should use pairwise comparisons or GLMs?
    That is because if I use GLMs, I assume no difference at time 1h, but I know that there are!
    On the other side if I use pairwise comparisons I lose the modulation through the time!
    I hope that my explanation is clear!
    Any ideas?

    Thank you

    Giuliano

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