I'm validating a MBD-seq library and having difficulty understanding the need for a non-enriched input sample. We're using a MACS based approach to analyse the library, which compares Naive and 4 treatment groups. Someone suggested to me that peaks need to be found relative to input DNA and then compared, but it appears that MACS generates a "local background" to find peaks so input may not be necessary. Could someone explain the benefit of an input sample? I prefer to use the naive profile as a control. Thanks!
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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