Hello everyone,
I am planning to prepare an Illumina library for the DNA/RNA hybridome of the cell. I already have the immuno-precipitated material, and I had a few questions before I begin library prep. I want to proceed in 2 ways-
a) digest the DNA in the sample (leaving just RNA) and perform a standard RNA-seq.
b) digest the RNA using RNase H and prepare the library using the ssDNA as starting material.
My question is regarding the second approach (b). Does anyone have any experience with making libraries using ssDNA as the starting sample?
Any other comments/suggestions are most welcome.
Thanks in advance.
I am planning to prepare an Illumina library for the DNA/RNA hybridome of the cell. I already have the immuno-precipitated material, and I had a few questions before I begin library prep. I want to proceed in 2 ways-
a) digest the DNA in the sample (leaving just RNA) and perform a standard RNA-seq.
b) digest the RNA using RNase H and prepare the library using the ssDNA as starting material.
My question is regarding the second approach (b). Does anyone have any experience with making libraries using ssDNA as the starting sample?
Any other comments/suggestions are most welcome.
Thanks in advance.
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