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Old 02-03-2014, 04:53 PM   #41
paa6
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I did change it...and will let u know what is he saying...still waiting for him...
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Old 02-03-2014, 05:11 PM   #42
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hoe so...I am waiting for PI an dI will discuss with him...hope so that he will understand everything...ok I will try to change the title...
HEY i DID talk to him and luckily he understood...thanks a ton...
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Old 02-03-2014, 09:37 PM   #43
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HEY i DID talk to him and luckily he understood...thanks a ton...
i HAVE ONE MORE que. geneious doesn't support .sff file format...
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Old 02-04-2014, 12:56 AM   #44
sklages
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Well, you have a SFF file with a missing manifest. Probably corrupted.

Again, ask your PI for the "complete" dataset. There went probably something wrong during transfer. For the SFF file check the MD5 sum before and after copy process, just to be sure, that it has been transferred completely.

And, yes, it seems that geneious does not support native SFF import :-(
But if you have the complete dataset you can just use use the fna/qual pair (always use the quality files if available), just as intented from the very beginning :-)

But of course, you can have a look at "sff_extract" or Roche's "SFF Tools" just to be able to deal with SFF files on the command line.

regards.
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Old 02-04-2014, 01:05 AM   #45
paa6
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Originally Posted by sklages View Post
Well, you have a SFF file with a missing manifest. Probably corrupted.

Again, ask your PI for the "complete" dataset. There went probably something wrong during transfer. For the SFF file check the MD5 sum before and after copy process, just to be sure, that it has been transferred completely.

And, yes, it seems that geneious does not support native SFF import :-(
But if you have the complete dataset you can just use use the fna/qual pair (always use the quality files if available), just as intented from the very beginning :-)

But of course, you can have a look at "sff_extract" or Roche's "SFF Tools" just to be able to deal with SFF files on the command line.

regards.
hmm ok thanks, I will try that..
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