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  • About identify the diffenential expression gene

    Hi,everyone
    Currently ,We use SOLID to study transcriptome . I want to ask how to identify the diffenential expression gene ,just use the result of RPKM or some models ? Sorry , I am a layman on the bioinformatics .\
    Thank you for your help ~

  • #2
    Hi,
    It's not easy... This article might give you some clues "Stem cell transcriptome profiling via massive-scale mRNA sequencing" Nature methods.

    Sheila

    Comment


    • #3
      @Ruby

      **Disclaimer: I work at CLC bio**
      Hi Ruby,

      We have some tutorials on expression analysis that may be of interest to you, you can find these at - http://www.clcbio.com/index.php?id=649

      We also have some videos that go through the analysis of expression data, step by step. You can find these at - http://clcbio.blip.tv/
      One of them is even in Chinese ;-)

      Hope this helps and best of luck on your research!!

      Cheers

      Roald

      **Disclaimer: I work at CLC bio**

      Comment


      • #4
        Thanks a lot ,Roald ,I will consult you if I have some problems, thanks again~~~

        Comment


        • #5
          Originally posted by Roald View Post
          **Disclaimer: I work at CLC bio**
          Hi Ruby,

          We have some tutorials on expression analysis that may be of interest to you, you can find these at - http://www.clcbio.com/index.php?id=649

          We also have some videos that go through the analysis of expression data, step by step. You can find these at - http://clcbio.blip.tv/
          One of them is even in Chinese ;-)

          Hope this helps and best of luck on your research!!

          Cheers

          Roald

          **Disclaimer: I work at CLC bio**


          HI,Roald ,I can't open the http://clcbio.blip.tv/ ,is this website working ? Can I have your MSN or some other tools that I can contact you? Mine: [email protected]

          Thanks ~~

          Comment


          • #6
            Hi Ruby,
            did you tried any of the links from here: http://www.clcbio.com/index.php?id=1342

            Stegger

            Comment


            • #7
              @Ruby, I have no problems loading the website, are you seeing the other pages alright. If you have further problems with the website, please contact [email protected]

              @Stegger, thanks for posting the link

              Cheers

              Roald

              Comment

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