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  • De novo Transcriptome and size selection

    Hello,
    I would like to have your opinion on this:
    Would it help me for the my transcriptome assembly if I first made a size selection during the RNA seq library construction ?
    the Pros and Cons I saw:
    Pro:
    This could allow to jump over repeated protein domain
    Reduce the possible small RNA contamination
    Cons:
    Lossing small mRNA fragements
    Make the library construction more complicated

    Thanks a lot for your answers

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