Hello, we are planning to make a TruSeq library with an starting insert of 500bp and worried that this higher molarity might affect the subsequent TruSeq kit rxns. The max range recommended in the manuel is 300bp insert.
Would multiplying the reagents and volumes by (500/300 ) = 1.667 be the best course? Or start with .67ug of the 500bp gDNA?
Does anyone have experience making TruSeq libraries this size?
thanks, ap
Would multiplying the reagents and volumes by (500/300 ) = 1.667 be the best course? Or start with .67ug of the 500bp gDNA?
Does anyone have experience making TruSeq libraries this size?
thanks, ap
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