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12-27-2016, 03:52 AM
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#1 |
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Junior Member
Location: Norway, Bergen Join Date: Nov 2016
Posts: 3
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On-Plate ATAC-seq
Hello,
Could anyone please drop any comments on libraries that i have prepared using on-plate ATAC protocol (DOI: 10.1186/s13059-015-0840-9). Libraries seem to be overtagmented, but i would like to receive any comment considering their sequencibility and further actions of optimising tagmentation protocol. This time i used standard NEXTERA protocol(2,5ul of enzyme) and 50000 HeLa cells. Thanks in advance. |
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02-23-2017, 04:26 AM
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#2 |
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Member
Location: UK Join Date: Jun 2012
Posts: 14
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Hi,
did you get any feedback on those libraries? I have got something similar but from different cells. Did you sequenced them at the end? if yes, how was the result? Thanks Dam |
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02-28-2017, 05:16 AM
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#3 |
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Junior Member
Location: Norway, Bergen Join Date: Nov 2016
Posts: 3
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No, those libraries haven`t been sent for sequencing. Instead we have produced another samples. The thing is they are the way too overtagmented, so go down with incubation time(even to 5-10 minutes) or try removing permeabilization step.
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| Tags |
| atac-seq, library preparation |
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