Hi,
I have 3 questions about RNA-seq analysis:
1. How do I know the inner distance between two pair-end seq? If the sequencing company don't tell me that, how can I find it.
2. If the inner distance is 100bp, but i trimmed the pair-end seq, so when I use tophat, how to set the inner distance, it is still 100bp?
3. How to calculate the standard deviation for inner distance, for tophat.
Thanks a lot!
I have 3 questions about RNA-seq analysis:
1. How do I know the inner distance between two pair-end seq? If the sequencing company don't tell me that, how can I find it.
2. If the inner distance is 100bp, but i trimmed the pair-end seq, so when I use tophat, how to set the inner distance, it is still 100bp?
3. How to calculate the standard deviation for inner distance, for tophat.
Thanks a lot!