TruSeq libraries have been generated, but are extremly low (about 0.3 ng/uL). I usually save half of my ligated product in case I need to go back to it. Is it possible to re-do A-tail step, and ligation of adapters, and then to PCR again, then pool with low original libraries.
Its seems as if ligation of adapters were not as efficient.
Its seems as if ligation of adapters were not as efficient.
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