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Old 03-30-2016, 12:00 AM   #1
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Location: Bangladesh

Join Date: Mar 2016
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Default Plasmid design using APE

Hey guys, I'm a newbie to bioinformatics and still doing an undergrad. But I really needed help with something and hope you guys can. I am designing an expression vector using APE and needed to know about how to engineer the ribosome binding site into it. I'm sorry if this is a really dumb question but like I said, complete newbie.
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Old 03-30-2016, 04:17 AM   #2
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Location: East Coast USA

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For this exercise you may want to resort to a low tech (pen and paper/talking this through with someone in person) method. Especially if you are new to informatics and do not have adequate biology background. Only you know what kind resources (vectors/fragments/enzymes) are available in the lab you are working in and unless you provide a lot of additional information it may not be possible for folks here to help.
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ape, plasmid, vectors

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