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Old 09-25-2012, 07:30 AM   #1
klebsiella
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Location: Heidelberg

Join Date: Jul 2010
Posts: 10
Default Multiplexing Transcription factor Chip-seq

Hi all,

We will be soon starting to do libraries from Transcription factor (TF) Chip-seq, for a genome of ~ 330 Mb.

We want to multiplex and sequence them on HiSeq. We are going to multiplex 10 samples together.

I would like to know if:

1) Multiplexing 10 samples is too much
2) I read that multiplexing TF chip-seq is not good (maybe on GAII though). Does this hold true for HiSeq ?
3) Do you have any recommendation to take into account ? protocols to read ? ...

Many thanks for all your help,
All best,
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chip-seq, hiseq, library generation, multiplexing

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