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Old 02-22-2010, 04:41 PM   #1
artem.men
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Location: Brisbane

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Default RNA seq libraries from few hundred cells

Dear all,
we have to make RNA seq GA libraries from ~500 mammalian cells. Are there good kits/methods/publications on how get enough material for sequencing? Has anybody in this community done something similar?
Thanks very much.
Art.
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Old 03-02-2010, 05:02 PM   #2
kainsteven
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Default RNA-Seq from 500 pg total RNA

We recently presented results at AGBT showing the use of our Ovation RNA-Seq System for low input studies such as you mention here. The input range for total RNA is from 500 pg to 10 ng, with no poly(A) selection or rRNA reduction required.

http://www.nugeninc.com/nugen/index....na-seq-system/

Steve
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Old 03-02-2010, 05:04 PM   #3
artem.men
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Default

Thanks Steve, I've heard some good things about your new kit.
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