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Old 10-18-2014, 10:44 AM   #1
Location: Heraklion, Greece

Join Date: Jan 2014
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Default demultiplexing with dual indexing

Hi all, I am trying ascertain that my libraries can be demultiplexed. What we have are 6 samples prepared with the Nextera XT kit (whole genome shotgun). They each have unique i7 indexes, but all have the same i5 index. Even if read1 and read2 do not overlap, the samples should still demultiplex fine, right? Probably a silly question, but I just want to be certain....
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Old 10-18-2014, 12:47 PM   #2
Brian Bushnell
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Yes, demultiplexing is unrelated to whether reads overlap.
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demultiplexing, dual indexing, miseq, nextera xt

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