Plasmid design using APE

Saam · 03-30-2016, 12:00 AM

Hey guys, I'm a newbie to bioinformatics and still doing an undergrad. But I really needed help with something and hope you guys can. I am designing an expression vector using APE and needed to know about how to engineer the ribosome binding site into it. I'm sorry if this is a really dumb question but like I said, complete newbie.

GenoMax · 03-30-2016, 04:17 AM

For this exercise you may want to resort to a low tech (pen and paper/talking this through with someone in person) method. Especially if you are new to informatics and do not have adequate biology background. Only you know what kind resources (vectors/fragments/enzymes) are available in the lab you are working in and unless you provide a lot of additional information it may not be possible for folks here to help.

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03-30-2016, 12:00 AM	#1
Saam Junior Member Location: Bangladesh Join Date: Mar 2016 Posts: 1	Plasmid design using APE Hey guys, I'm a newbie to bioinformatics and still doing an undergrad. But I really needed help with something and hope you guys can. I am designing an expression vector using APE and needed to know about how to engineer the ribosome binding site into it. I'm sorry if this is a really dumb question but like I said, complete newbie.

03-30-2016, 04:17 AM	#2
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,087	For this exercise you may want to resort to a low tech (pen and paper/talking this through with someone in person) method. Especially if you are new to informatics and do not have adequate biology background. Only you know what kind resources (vectors/fragments/enzymes) are available in the lab you are working in and unless you provide a lot of additional information it may not be possible for folks here to help.