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Similar Threads
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02-08-2017, 06:23 AM
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#1 |
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Member
Location: Montreal Join Date: Jan 2017
Posts: 13
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Maximum number of cycles for kit Miseq 50 cycles
Hi sequencing Masters!
What is the maximum number of cycles we can do with a Miseq V2 50 cycles kit. I have been told that there is enough reagents for more than 55 cycles including the 6 cycles for index reading. Can someone confirm this information? Thank you very much for your support. It is very appreciated. |
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02-08-2017, 06:26 AM
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#2 |
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Senior Member
Location: East Coast USA Join Date: Feb 2008
Posts: 7,091
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How many are you looking to get?
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02-08-2017, 06:28 AM
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#3 |
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Member
Location: Montreal Join Date: Jan 2017
Posts: 13
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54 maximum + 6 for index
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02-08-2017, 06:32 AM
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#4 |
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Senior Member
Location: East Coast USA Join Date: Feb 2008
Posts: 7,091
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It should be fine. Local core does 66 cycle (50x8x8) runs off and on.
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02-08-2017, 06:48 AM
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#5 |
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Member
Location: Montreal Join Date: Jan 2017
Posts: 13
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Great news. Thank you for your answer Genomax!
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02-08-2017, 11:33 AM
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#6 |
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Member
Location: Montreal Join Date: Jan 2017
Posts: 13
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I got an answer from Illumina's tech support:
MiSeq v2 contains enough reagents for 75 cycles of chemistry |
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02-14-2017, 09:34 PM
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#7 |
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Junior Member
Location: China Join Date: Oct 2015
Posts: 1
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10-18-2017, 09:03 PM
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#8 |
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Junior Member
Location: washington Join Date: Jan 2016
Posts: 3
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I know this is late but we routinely do 78 cycles with the MiSeq v2 50-cycle kit. (31 read1, 31 read2, 8 index1, 8 index2).
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10-18-2017, 10:28 PM
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#9 |
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Jafar Jabbari
Location: Melbourne Join Date: Jan 2013
Posts: 1,238
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Plus 7 dark cycles used for index2 read so 85 cycles in total. This seems to be 10 cycles more than Illumina specification.
Last edited by nucacidhunter; 10-18-2017 at 10:30 PM. |
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