Create local BLAST database

SeqClark · 03-05-2011, 10:16 PM

Hi there,

I tried to use makeblastdb to create a local BLAST database and got the following error message.

I checked the FASTA format of my file and it seemed to be OK. No idea how to correct this problem. Any help would be greatly appreciated.

"Error: NCBI C++ Exception:
"..\..\..\..\..\src\serial\objistrasnb.cpp", line 164: Error: ncbi::CObjectI
StreamAsnBinary::UnexpectedTagClassByte() - byte 0: unexpected tag class/constru
cted: #255, should be #160 ( at Seq-entry)"

SeqClark · 03-05-2011, 10:20 PM

FAST sequence sample

>gi|157676334|ref|NP_067082.2| transmembrane protein 8A precursor [Homo sapiens]
MGRAGTGTGGEAVAAVVAGPLLLLLLARPPPASAGYSGKSEVGLVSEHFSQAPQRLSFYSWYGSARLFRF
RVPPDAVLLRWLLQVSRESGAACTDAEITVHFRSGAPPVINPLGTSFPDDTAVQPSFQVGVPLSTTPRSN
ASVNVSHPAPGDWFVAAHLPPSSQKIELKGLAPTCAYVFQPELLVTRVVEISIMEPDVPLPQTLLSHPSY
LKVFVPDYTRELLLELRDCVSNGSLGCPVRLTVGPVTLPSNFQKVLTCTGAPWPCRLLLPSPPWDRWLQV
TAESLVGPLGTVAFSAVAALTACRPRSVTIQPLLQSSQNQSFNASSGLLSPSPDHQDLGRSGRVDRSPFC
LTNYPVTREDMDVVSVHFQPLDRVSVRVCSDTPSVMRLRLNTGMDSGGSLTISLRANKTEMRNETVVVAC
VNAASPFLGFNTSLNCTTAFFQGYPLSLSAWSRRANLIIPYPETDNWYLSLQLMCPENAEDCEQAVVHVE
TTLYLVPCLNDCGPYGQCLLLRRHSYLYASCSCKAGWRGWSCTDNSTAQTVAQQRAATLLLTLSNLMFLA
PIAVSVRRFFLVEASVYAYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGAAIWVTILCMARLKT
VLKYVLFLLGTLVIAMSLQLDRRGMWNMLGPCLFAFVIMASMWAYRCGHRRQCYPTSWQRWAFYLLPGVS
MASVGIAIYTSMMTSDNYYYTHSIWHILLAGSAALLLPPPDQPAEPWACSQKFPCHYQICKNDREELYAV
T

rglover · 03-07-2011, 02:17 AM

What version of the NCBI executables are you using? What command line arguments are you using to create your database?

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03-05-2011, 10:16 PM	#1
SeqClark Junior Member Location: NJ Join Date: Mar 2011 Posts: 2	Create local BLAST database Hi there, I tried to use makeblastdb to create a local BLAST database and got the following error message. I checked the FASTA format of my file and it seemed to be OK. No idea how to correct this problem. Any help would be greatly appreciated. "Error: NCBI C++ Exception: "..\..\..\..\..\src\serial\objistrasnb.cpp", line 164: Error: ncbi::CObjectI StreamAsnBinary::UnexpectedTagClassByte() - byte 0: unexpected tag class/constru cted: #255, should be #160 ( at Seq-entry)"

03-05-2011, 10:20 PM	#2
SeqClark Junior Member Location: NJ Join Date: Mar 2011 Posts: 2	FAST sequence sample >gi\|157676334\|ref\|NP_067082.2\| transmembrane protein 8A precursor [Homo sapiens] MGRAGTGTGGEAVAAVVAGPLLLLLLARPPPASAGYSGKSEVGLVSEHFSQAPQRLSFYSWYGSARLFRF RVPPDAVLLRWLLQVSRESGAACTDAEITVHFRSGAPPVINPLGTSFPDDTAVQPSFQVGVPLSTTPRSN ASVNVSHPAPGDWFVAAHLPPSSQKIELKGLAPTCAYVFQPELLVTRVVEISIMEPDVPLPQTLLSHPSY LKVFVPDYTRELLLELRDCVSNGSLGCPVRLTVGPVTLPSNFQKVLTCTGAPWPCRLLLPSPPWDRWLQV TAESLVGPLGTVAFSAVAALTACRPRSVTIQPLLQSSQNQSFNASSGLLSPSPDHQDLGRSGRVDRSPFC LTNYPVTREDMDVVSVHFQPLDRVSVRVCSDTPSVMRLRLNTGMDSGGSLTISLRANKTEMRNETVVVAC VNAASPFLGFNTSLNCTTAFFQGYPLSLSAWSRRANLIIPYPETDNWYLSLQLMCPENAEDCEQAVVHVE TTLYLVPCLNDCGPYGQCLLLRRHSYLYASCSCKAGWRGWSCTDNSTAQTVAQQRAATLLLTLSNLMFLA PIAVSVRRFFLVEASVYAYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGAAIWVTILCMARLKT VLKYVLFLLGTLVIAMSLQLDRRGMWNMLGPCLFAFVIMASMWAYRCGHRRQCYPTSWQRWAFYLLPGVS MASVGIAIYTSMMTSDNYYYTHSIWHILLAGSAALLLPPPDQPAEPWACSQKFPCHYQICKNDREELYAV T

03-07-2011, 02:17 AM	#3
rglover rg Location: uk Join Date: Dec 2008 Posts: 51	What version of the NCBI executables are you using? What command line arguments are you using to create your database?