Dear everyone,
Im preparing a RRBS library. I want to multiplex 10 samples from 150ng DNA.
We use ilumnia DNA nano LT adaptors on the fragmented DNA.
I have been looking online for multiplexing guidelines, but cannot seem to find anything on what is recommended.
I used these adaptors:
TRUSEQ_1 ATCACG
TRUSEQ_2 CGATGT
TRUSEQ_4 TGACCA
TRUSEQ_5 ACAGTG
TRUSEQ_6 GCCAAT
TRUSEQ_7 CAGATC
TRUSEQ_8 ACTTGA
TRUSEQ_10 TAGCTT
TRUSEQ_11 GGCTAC
TRUSEQ_12 CTTGTA
Would this multiplexing with the converted DNA cause too low diversity of clusters or could it be manageable?
We would spike in with 30% PhiX as allways to reduce cluster diversity.
We use a protocol similar to this:
Thank you for your help.
Im preparing a RRBS library. I want to multiplex 10 samples from 150ng DNA.
We use ilumnia DNA nano LT adaptors on the fragmented DNA.
I have been looking online for multiplexing guidelines, but cannot seem to find anything on what is recommended.
I used these adaptors:
TRUSEQ_1 ATCACG
TRUSEQ_2 CGATGT
TRUSEQ_4 TGACCA
TRUSEQ_5 ACAGTG
TRUSEQ_6 GCCAAT
TRUSEQ_7 CAGATC
TRUSEQ_8 ACTTGA
TRUSEQ_10 TAGCTT
TRUSEQ_11 GGCTAC
TRUSEQ_12 CTTGTA
Would this multiplexing with the converted DNA cause too low diversity of clusters or could it be manageable?
We would spike in with 30% PhiX as allways to reduce cluster diversity.
We use a protocol similar to this:
Thank you for your help.
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