Hi everyone,
Please forgive my naive questions, I am just begining to work with NGS. I need to modify the blunt end step to incorporate repair enzymes (UDG and Endo VIII as described in one of the Neanderthal NGS papers).
I try to understand exactly what is going on during the blunt end and I wonder why we need the Klenow pol since the T4 DNA Pol has a 3'-5' exonuclease activity? Is it to fill up nicks?
Please forgive my naive questions, I am just begining to work with NGS. I need to modify the blunt end step to incorporate repair enzymes (UDG and Endo VIII as described in one of the Neanderthal NGS papers).
I try to understand exactly what is going on during the blunt end and I wonder why we need the Klenow pol since the T4 DNA Pol has a 3'-5' exonuclease activity? Is it to fill up nicks?
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