Hello,
I am trying to design an experiment :
and my question is can I put together my 3 reps of RNA before doing the library and then sequence the 3 reps as one. I will compare to a non treated samples and to different times points
ending with 6 libraries instead of 18.
I am looking only for very strong differences I can then verify my datas by qPCR on the single samples.
1-Is there any problem with this design?
2- what am I loosing against doing separate libraries for each reps beside the statistical ?
thanks
I am trying to design an experiment :
and my question is can I put together my 3 reps of RNA before doing the library and then sequence the 3 reps as one. I will compare to a non treated samples and to different times points
ending with 6 libraries instead of 18.
I am looking only for very strong differences I can then verify my datas by qPCR on the single samples.
1-Is there any problem with this design?
2- what am I loosing against doing separate libraries for each reps beside the statistical ?
thanks
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