hi, I havre constructed the small RNA library following the exact protocol of ILMN TruSeq small RNA with ILMN small RNA kit. and excised the bands between 145bp and 160bp, but the agilent 2200 showed the library was only 135bp!(see the attachment), and so as other samples!All samples were human samples.
Did I cut the wrong band? I already try the upper band(B band), and the 2200 result was 172bp. Which band is the right library. This was the first trial experiment in the lab, and we really need help before running.
Thanks in advance!
Tanya
Did I cut the wrong band? I already try the upper band(B band), and the 2200 result was 172bp. Which band is the right library. This was the first trial experiment in the lab, and we really need help before running.
Thanks in advance!
Tanya
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