Hi all,
We have been preparing libraries for Illumina sequencing using the ScriptSeq v2 kit. This gave us a bit of trouble and the resulting library is not really perfect. There is a large peak at 69 bp.
Please see the bioanalyzer image.
The peak at 69 bp is about the size of a single primer. Not an adapter-dimer.
My question is would this be a problem for cluster generation and sequencing?
This type of contamination would not be amplified, so I guess it would not be a problem, but I might be missing something? Would you feel safe sequencing this library?
Thank you,
Morten.
We have been preparing libraries for Illumina sequencing using the ScriptSeq v2 kit. This gave us a bit of trouble and the resulting library is not really perfect. There is a large peak at 69 bp.
Please see the bioanalyzer image.
The peak at 69 bp is about the size of a single primer. Not an adapter-dimer.
My question is would this be a problem for cluster generation and sequencing?
This type of contamination would not be amplified, so I guess it would not be a problem, but I might be missing something? Would you feel safe sequencing this library?
Thank you,
Morten.
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