We have some older runs we did with SCS 2.6 and CASAVA 1.6. We no longer have the qseq or pretty much any other files, but we do have fastqs. Unfortunately, the fastqs were not filtered for the reads that passed the chastity filter, and that data is not in the fastq file itself.
Does anyone know where I can find the documentation that has the criteria SCS 2.6 used to tell if a read passed filter so that I can write a script to re-evaluate the reads in the fastq files? Or does anyone know of another solution to making these fastq files usable again?
Thanks for the help.
Does anyone know where I can find the documentation that has the criteria SCS 2.6 used to tell if a read passed filter so that I can write a script to re-evaluate the reads in the fastq files? Or does anyone know of another solution to making these fastq files usable again?
Thanks for the help.
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