Hi Seqanswers community,
I going to test size selection step in Pippin-Prep and I´m not sure about the advantages and disadvantages to use the external or internal markers.
I going to use a 2% dye-free Pippin-Prep cassete gel to select two different ranges, 200-250 and 400-450 bp. The marker options for my experiment are Marker L (internal standards) or Marker K (external standards).
I have already performed a test using the external Marker K to select the above mentioned ranges and using the 'Range Program Mode'. However, the product size recovered was smaller than selected (around 150 bp for the 200-250 range and around 350 for the range 400-450). I guess if I use a internal instead external marker I can improve accuracy, but I afraid that I can collect a marker fragment togheter with the recovered product.
Thank you.
I going to test size selection step in Pippin-Prep and I´m not sure about the advantages and disadvantages to use the external or internal markers.
I going to use a 2% dye-free Pippin-Prep cassete gel to select two different ranges, 200-250 and 400-450 bp. The marker options for my experiment are Marker L (internal standards) or Marker K (external standards).
I have already performed a test using the external Marker K to select the above mentioned ranges and using the 'Range Program Mode'. However, the product size recovered was smaller than selected (around 150 bp for the 200-250 range and around 350 for the range 400-450). I guess if I use a internal instead external marker I can improve accuracy, but I afraid that I can collect a marker fragment togheter with the recovered product.
Thank you.
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