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  • #1

    HiC - crosslinking - glycine

    hello. i have 2 questions.

    1. what would be the best way to evaluate if the cross-linking went okay for Hi-C (chromosome interactions)?

    2. i read different things about the temperature of quenching the formaldehyde with glycine. some do it at RT, some do it on ice or with ice-cold glycine. i am not a chemist. why would the temperature be of importance?

    thanks for reading the post and your kind help!

    Seb
    Tags: crosslinking, formaldehyde, glycine, hic
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