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Old 07-15-2013, 10:44 AM   #1
Location: Istanbul, Turkey

Join Date: Oct 2012
Posts: 28
Default What to do after finding differentially expressed genes?

Hello all,

Which programs do you use after finding the differentially expressed genes between 2 conditions? I assume, everyone first look at which GO terms are enriched. So far I have used DAVID, attempted to use GSEA (but did not work well, maybe I am doing something wrong). Also I will be happy if anyone can inform me how to arrange the files in GSEA for RNA-seq.

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Old 07-15-2013, 11:39 AM   #2
Devon Ryan
Location: Freiburg, Germany

Join Date: Jul 2011
Posts: 3,480

There's GOSeq, which is probably a bit more appropriate for RNAseq datasets than DAVID. Aside from that, it depends on the biological question.
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