The Best Robot for NGS Library Prep

gkuffel · 09-30-2015, 09:20 AM

Hi everyone,

I wanted to get some insight on a robot for NGS library prep. I am a Lab Mgr. of a NGS core that currently runs 2 MiSeqs. So far we have been considering the EpMotion 5075 but it is quite expensive and I wanted to get some insight from some folks that may have experience integrating these types of robots into their workflows. Thanks for your help.

Bukowski · 10-01-2015, 12:57 AM

Have you considered the NGS Express?

http://www.perkinelmer.co.uk/Catalog...%20Workstation

We're pretty high throughput so do most of our library prep on Sciclones, but the NGS Express still gets quite a bit of use for lower throughput projects in the lab. Should handle most of your critical MiSeq applications.

Angelmass · 10-13-2015, 03:04 PM

We use Biomek FXs and Hamilton Star and Starlets, but I don't know if that would really solve your cost problem, since they're both pretty large and expensive-looking. Another consideration you might have to take into account is whether or not you need automation personnel for support.
We're not super high throughput though, about 100 samples a day.

DRYTCYV · 10-14-2015, 05:53 AM

Is there any liquid handling users that can give some feedback?
Its for a genomic core facility with a Illumina platform technology (Miseq, NextSeq or HiSeq).

epMotion 5075 vs Hamilton STAR vs Sciclone NGS.

-Consumables
-Optimization

Thanks in advance

ScienceGrrl · 10-20-2015, 08:05 AM

I work for Hamilton Robotics as the Scientific Leader for NGS, but came from Beckman Coulter where I was the application scientist developing the NGS methods for them. If you would like to PM me and ask specific questions about automating NGS protocols, I can answer them.

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09-30-2015, 09:20 AM	#1
gkuffel Genomics Lab Mgr. Location: Chicago Join Date: Oct 2012 Posts: 14	The Best Robot for NGS Library Prep Hi everyone, I wanted to get some insight on a robot for NGS library prep. I am a Lab Mgr. of a NGS core that currently runs 2 MiSeqs. So far we have been considering the EpMotion 5075 but it is quite expensive and I wanted to get some insight from some folks that may have experience integrating these types of robots into their workflows. Thanks for your help.

10-01-2015, 12:57 AM	#2
Bukowski Senior Member Location: UK Join Date: Jan 2010 Posts: 390	Have you considered the NGS Express? http://www.perkinelmer.co.uk/Catalog...%20Workstation We're pretty high throughput so do most of our library prep on Sciclones, but the NGS Express still gets quite a bit of use for lower throughput projects in the lab. Should handle most of your critical MiSeq applications.

10-13-2015, 03:04 PM	#3
Angelmass Member Location: California Join Date: Jun 2015 Posts: 10	We use Biomek FXs and Hamilton Star and Starlets, but I don't know if that would really solve your cost problem, since they're both pretty large and expensive-looking. Another consideration you might have to take into account is whether or not you need automation personnel for support. We're not super high throughput though, about 100 samples a day.

10-14-2015, 05:53 AM	#4
DRYTCYV Member Location: Portugal Join Date: Apr 2011 Posts: 50	Is there any liquid handling users that can give some feedback? Its for a genomic core facility with a Illumina platform technology (Miseq, NextSeq or HiSeq). epMotion 5075 vs Hamilton STAR vs Sciclone NGS. -Consumables -Optimization Thanks in advance

10-20-2015, 08:05 AM	#5
ScienceGrrl Junior Member Location: Indianapolis, IN Join Date: Jul 2015 Posts: 8	I work for Hamilton Robotics as the Scientific Leader for NGS, but came from Beckman Coulter where I was the application scientist developing the NGS methods for them. If you would like to PM me and ask specific questions about automating NGS protocols, I can answer them.