Does small RNA sequencing generate comparable reads as regular Solexa sequencing?

Goodluck · 08-07-2009, 08:25 PM

Hi,

I am curious whether small RNA sequencing will generate similar numbers of reads as the regular single-end Solexa sequencing. Thanks!

chris · 08-19-2009, 02:12 AM

Yup. It's just that you'll get far more depth than for genomic/transcriptomic sequencing i.e. >100K copies of the same sequence. This is due to the sample being less complex.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Small RNA sequencing] 10-12 M raw reads enough?	harrike	SOLiD	0	12-03-2010 01:35 PM
[Small RNA sequencing] 10-12 M raw reads enough?	harrike	454 Pyrosequencing	4	12-03-2010 01:34 PM
How to trim the adaptor sequence from the solexa small RNA sequencing data?	satp	Bioinformatics	11	11-17-2010 02:08 PM
Calculating small RNA expressions from Solexa Sequencing with Cufflinks	DrD2009	Bioinformatics	6	06-29-2010 08:12 PM
Longer reads for small RNA sequencing?	Goodluck	Illumina/Solexa	3	03-31-2009 06:58 AM

08-07-2009, 08:25 PM	#1
Goodluck Junior Member Location: Canada Join Date: Mar 2009 Posts: 3	Does small RNA sequencing generate comparable reads as regular Solexa sequencing? Hi, I am curious whether small RNA sequencing will generate similar numbers of reads as the regular single-end Solexa sequencing. Thanks!

08-19-2009, 02:12 AM	#2
chris Member Location: Dundee, Scotland Join Date: Apr 2008 Posts: 52	Yup. It's just that you'll get far more depth than for genomic/transcriptomic sequencing i.e. >100K copies of the same sequence. This is due to the sample being less complex.