Any one have been compared the fragment method between nebulizer and covaris ？

stare024 · 08-09-2012, 01:11 AM

which is better？

Aniki · 08-14-2012, 06:53 PM

I have never used a nebulizer, but I do use Covaris. I get good libraries with Covaris.

jinpingzhang · 08-14-2012, 07:00 PM

Quote:

Originally Posted by stare024

which is better？

Yes, i think Cavaris should be better

RCJK · 08-14-2012, 07:19 PM

I have used the Covaris for 454 libraries to be sequenced with the XLR70 kit with success, but have not had luck getting the sizing right for longer fragment libraries to be sequenced on the XL+ kit. I believe 454 is working on this, but the parameters they suggested I use gave me library fragments that were too large and they recommended I not sequence them.

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08-09-2012, 01:11 AM	#1
stare024 Junior Member Location: suzhou city china Join Date: Apr 2012 Posts: 4	Any one have been compared the fragment method between nebulizer and covaris ？ which is better？

08-14-2012, 06:53 PM	#2
Aniki Member Location: Toronto Join Date: Mar 2011 Posts: 39	I have never used a nebulizer, but I do use Covaris. I get good libraries with Covaris.

08-14-2012, 07:19 PM	#4
RCJK Senior Member Location: Australia Join Date: May 2009 Posts: 155	I have used the Covaris for 454 libraries to be sequenced with the XLR70 kit with success, but have not had luck getting the sizing right for longer fragment libraries to be sequenced on the XL+ kit. I believe 454 is working on this, but the parameters they suggested I use gave me library fragments that were too large and they recommended I not sequence them.