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08-12-2011, 05:22 AM
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#1 |
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Senior Member
Location: uk Join Date: Jan 2010
Posts: 110
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Coverage required for Sanger based SNP detection and Genotyping
Does anyone know of an established acceptable figure in terms of times coverage required for SNP/calling and genotyping with Sanger sequences?
I know 20x is generally recommended for NGS but this is obviously a different scenario. |
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08-30-2011, 12:54 AM
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#2 |
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Member
Location: china Join Date: Dec 2010
Posts: 12
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many papers using the Sanger sequences ,but didn't give a criteria . maybe 10x is enough.
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08-30-2011, 01:33 AM
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#3 |
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Member
Location: Edinburgh Join Date: Mar 2010
Posts: 16
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We take 2x coverage for SNP calling in our lab.
Ventner's genome was done to 7.5x (http://www.nature.com/nrg/journal/v1...df/nrg2626.pdf) Cheers Paul |
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08-30-2011, 01:41 AM
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#4 |
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Member
Location: china Join Date: Dec 2010
Posts: 12
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Did you get a good result ? what's FP and FN ?
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