SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > SOLiD



Similar Threads
Thread Thread Starter Forum Replies Last Post
Good Beads / Best Beads paolo.kunder SOLiD 2 03-27-2013 03:12 PM
no enrichment of beads --> any ideas why? lichtfaengerin 454 Pyrosequencing 1 08-11-2011 03:45 AM
Calibration beads mestro2 Sample Prep / Library Generation 1 11-30-2010 08:59 AM
mag beads? link1 Sample Prep / Library Generation 1 08-13-2010 07:34 AM
Beads/slide Katsu SOLiD 1 09-22-2009 02:14 PM

Reply
 
Thread Tools
Old 06-14-2012, 07:07 AM   #1
quark
Member
 
Location: Slovakia

Join Date: Apr 2012
Posts: 10
Default P1 beads

Hello everybody,

can anyone tell me whether the concentration of P1 beads within P1 reagent (Box 2, SOLiD EZ Bead Emulsifier) is the same as that of P1 beads of ePCR Kit V2, that should be 10,000,000 beads /ul? As far as we use the EZ Bead system and not the IKA shaker we´ve got only 3 boxes for emulsifier (P1 beads included for DNA enrichment and not separate P1 beads for calculation of enriched P1beads conc.). Can I prepare the calibration curve from the remaining P1 beads assuming 10 mil.beads/ul concentration, too?thanks.
quark is offline   Reply With Quote
Old 07-09-2012, 01:40 AM   #2
JPC
Senior Member
 
Location: Wales

Join Date: May 2008
Posts: 114
Default

I'm not sure why you want to prepare a calibration curve? the EZ bead system doesn't require one?

I realise this is not really the answer you are looking for but you can easily find the concentration of a give tube, just prepare a serial dilution then your usual method for bead counting....I'd recommend using a nanodrop rather than the "colour chart".

JPC
JPC is offline   Reply With Quote
Old 08-15-2012, 08:41 PM   #3
jinpingzhang
Member
 
Location: Houston

Join Date: Mar 2012
Posts: 10
Default

As my calculate, i think that is true, 10millinon/ul, EZ80 usually use 1430ul x 10millions. method should be same like IKA utilized, which apply 160ul same concentration beads. AB maybe give you more information for this question.
jinpingzhang is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:57 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO