P1 beads

quark · 06-14-2012, 07:07 AM

Hello everybody,

can anyone tell me whether the concentration of P1 beads within P1 reagent (Box 2, SOLiD EZ Bead Emulsifier) is the same as that of P1 beads of ePCR Kit V2, that should be 10,000,000 beads /ul? As far as we use the EZ Bead system and not the IKA shaker we´ve got only 3 boxes for emulsifier (P1 beads included for DNA enrichment and not separate P1 beads for calculation of enriched P1beads conc.). Can I prepare the calibration curve from the remaining P1 beads assuming 10 mil.beads/ul concentration, too?thanks.

JPC · 07-09-2012, 01:40 AM

I'm not sure why you want to prepare a calibration curve? the EZ bead system doesn't require one?

I realise this is not really the answer you are looking for but you can easily find the concentration of a give tube, just prepare a serial dilution then your usual method for bead counting....I'd recommend using a nanodrop rather than the "colour chart".

JPC

jinpingzhang · 08-15-2012, 08:41 PM

As my calculate, i think that is true, 10millinon/ul, EZ80 usually use 1430ul x 10millions. method should be same like IKA utilized, which apply 160ul same concentration beads. AB maybe give you more information for this question.

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06-14-2012, 07:07 AM	#1
quark Member Location: Slovakia Join Date: Apr 2012 Posts: 10	P1 beads Hello everybody, can anyone tell me whether the concentration of P1 beads within P1 reagent (Box 2, SOLiD EZ Bead Emulsifier) is the same as that of P1 beads of ePCR Kit V2, that should be 10,000,000 beads /ul? As far as we use the EZ Bead system and not the IKA shaker we´ve got only 3 boxes for emulsifier (P1 beads included for DNA enrichment and not separate P1 beads for calculation of enriched P1beads conc.). Can I prepare the calibration curve from the remaining P1 beads assuming 10 mil.beads/ul concentration, too?thanks.

07-09-2012, 01:40 AM	#2
JPC Senior Member Location: Wales Join Date: May 2008 Posts: 114	I'm not sure why you want to prepare a calibration curve? the EZ bead system doesn't require one? I realise this is not really the answer you are looking for but you can easily find the concentration of a give tube, just prepare a serial dilution then your usual method for bead counting....I'd recommend using a nanodrop rather than the "colour chart". JPC

08-15-2012, 08:41 PM	#3
jinpingzhang Member Location: Houston Join Date: Mar 2012 Posts: 10	As my calculate, i think that is true, 10millinon/ul, EZ80 usually use 1430ul x 10millions. method should be same like IKA utilized, which apply 160ul same concentration beads. AB maybe give you more information for this question.