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07-19-2013, 06:24 AM
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Junior Member
Location: Germany Join Date: May 2013
Posts: 2
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homogenization of heart tissue for ChIPSeq
Hi,
I am trying doing ChIPSeq for mouse heart homogenates (100mg). I changed the protocol conditions for different times, but I always have the same problem, that I see two peaks in the Agilent Bioanalyzer, the first required peak with ca.200bp and a big peak at >2kb. I looked for different Cycle conditions, but I guess that I get mistakes in my sample preparation. How can I homogenize and handle the heart tissue in the right way? Thanks! |
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