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  • rRNA sizes on Agarose gel

    Hi All,

    I ran a Agarsoe gel with my RNA samples and got the attached pattern.
    23S rRNA and 16S rRNA are supposed to be ~3000 bps and ~1500 bps, however in this run I see 1500 and 750 bands.

    Can this be a gel problem? Has anyone encountered this?

    Thanks,

    Lior
    Attached Files

  • #2
    RNA will not run on a gel exactly according to its size because it is single stranded and the secondary structure can be variable. To get an accurate size you need to use a denaturing gel (formaldehyde, urea, methyl mercury) and compare it to RNA standards.

    Since you see 2 pretty sharp bands, your RNA is probably fine.

    Edit: There is also a bioanalyzer chip for RNA that would be an option if you have access to an instrument.
    Last edited by cliffbeall; 07-19-2013, 05:24 AM.

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