|
|
|
|
|||||||
Similar Threads
|
||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Maximum input material for Nextera library prep? | vramani | Illumina/Solexa | 0 | 08-22-2013 04:23 PM |
| Library Prep with Low Input | Joltex | Ion Torrent | 6 | 12-11-2012 01:13 PM |
| Library prep woes – unusual bioanalyzer profile of Illumina RNA-seq library | petey | Sample Prep / Library Generation | 1 | 11-19-2012 10:00 AM |
| Different PCR signal from input control after library prep | AveragePhD | Sample Prep / Library Generation | 14 | 10-17-2012 05:47 PM |
| Library prep for 50ng input with TruSeq DNA- or RNAseq adapters? | sehrrot | Sample Prep / Library Generation | 1 | 11-09-2011 05:03 AM |
 |
|
|
Thread Tools |
10-11-2013, 07:03 AM
|
#1 |
|
Junior Member
Location: UK Join Date: Mar 2013
Posts: 7
|
PacBio RS II Library Prep Input
Hi all,
I'm looking to sequence low quantities of PCR amplicon using the PacBio RS II system. I am trying to detect single nucleotide variants within these amplicons so need to minimise the number of PCR cycles I do (to avoid PCR-induced error). So I am looking to see if anyone knows what the input DNA template concentration requirement is for the library preparation kit 2.0 (250bp-->3kb)? Thanks, Joe |
|
|
|
10-15-2013, 03:32 PM
|
#2 |
|
Member
Location: Menlo Park, CA Join Date: Sep 2011
Posts: 87
|
http://www.smrtcommunity.com/SampleNet/Sample-Prep
there are a number of different protocols available for download. Roughly, 250bp requires 250ng. |
|
|
|
|
| Tags |
| library prep, pacbio, pacific biosciences, pcr amplicons |
| Thread Tools | |
|
|
