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Old 10-11-2013, 07:03 AM   #1
Junior Member
Location: UK

Join Date: Mar 2013
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Default PacBio RS II Library Prep Input

Hi all,

I'm looking to sequence low quantities of PCR amplicon using the PacBio RS II system. I am trying to detect single nucleotide variants within these amplicons so need to minimise the number of PCR cycles I do (to avoid PCR-induced error).

So I am looking to see if anyone knows what the input DNA template concentration requirement is for the library preparation kit 2.0 (250bp-->3kb)?


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Old 10-15-2013, 03:32 PM   #2
Location: Menlo Park, CA

Join Date: Sep 2011
Posts: 87

there are a number of different protocols available for download.

Roughly, 250bp requires 250ng.
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library prep, pacbio, pacific biosciences, pcr amplicons

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