Hi guys,
I've been dealing with RNA-seq count data consisting of several technical replicates.
And I tried to normalize the data using DESeq.
However, the DESeq author suggested to apply DESeq to biological replicates not to technical replicates.
Anyone knows the reason for this??
Why can't I apply DESeq normalization to my technical replicates????
Also, is there any method to normalize a technical replicates effectively??
Thank you for reading.
I've been dealing with RNA-seq count data consisting of several technical replicates.
And I tried to normalize the data using DESeq.
However, the DESeq author suggested to apply DESeq to biological replicates not to technical replicates.
Anyone knows the reason for this??
Why can't I apply DESeq normalization to my technical replicates????
Also, is there any method to normalize a technical replicates effectively??
Thank you for reading.
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