We build two different cDNA library for the same sample with different size(196 and 225) for pair end sequencing by Hiseq with the same sequence length 126. Then we map the two set of sequencing data to the genome by tophat. But the result show that the insert size for 225bp cDNA library is only 200bp, so whats the matter of it? How can I deal with it?
Thanks a lot for any possible reply.
Thanks a lot for any possible reply.
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